Preliminary Investigation of Different Types of Inoculums and Substrate Preparation for Biohydrogen Production

A pre-culture stage is required to obtain robustly-dividing cells with high hydrogen (H2) production capabilities. However, a step-by-step process for biohydrogen scarcely reported, mainly from palm oil wastewater. Therefore, this study developed guideline find the best inoculum heat treatment conditions and implement selected using This used raw mill effluent (POME) POME sludge as substrate inoculum, respectively. Our findings reveal that 80 °C 30 min were treatment. When testing on inoculating (28 g COD/L) at 37 (reaction temperature), 24 h time), pH 5.5, 34 mL H2/d was recorded. slight increase (1.1-fold) observed compared 5 COD/L co-digested g/L glucose (31 H2/d). discovery indicates potential source under anaerobic fermentation can be directly up COD/L. The proposed could also implemented different organic wastes study.